SLAMF1 ELISA 试剂盒
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- 抗原 See all SLAMF1 ELISA试剂盒
- SLAMF1 (Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: CD 150, Cd150, CD150 antigen, Cdw150, Estm51, Ipo 3, IPO-3, Ipo3, Signaling lymphocytic activation molecule, Signaling lymphocytic activation molecule family member 1, Signaling lymphocytic activation molecule family member 1 isoform CRA_a, Signaling lymphocytic activation molecule family member 1 isoform CRA_b, Signaling lymphocytic activation molecule precursor, Slaf1, SLAF1_HUMAN, SLAM family member 1, Slamf 1, Slamf1, SLAMF1 protein
Background: Signaling lymphocytic activation molecule is a protein that in humans is encoded by the SLAMF1 gene. It belongs to the immunoglobulin gene superfamily. This gene is mapped to 1q23.3. It has found that SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes and a proportion of B cells, and is rapidly induced on naive T cells after activation. In MV-resistant cell lines, infection with clinical MV and expression of SLAM, but not CD46, caused cytopathic effects (CPE). The expression of SLAM on activated B and T lymphocytes correlates with the pathology of MV infection in humans and monkeys, in which lymphoid organs are the chief sites of MV replication and the binding of MV to SLAM may impair the signaling functions of SLAM in lymphocyte activation and inhibit Th0/Th1 cytokine production, thereby promoting Th2 cytokine production. It has reported that antibody-mediated ligation of SLAM on thymocytes triggered a protein tyrosine phosphorylation signal in T cells in a SAP-dependent manner. This signal also involved SHIP, the adaptor molecules DOK2, DOK1, and SHC and RASGAP.
Gene Name: SLAMF1
Production: Natural and recombinant human SLAM. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: A21-K236
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human SLAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human SLAM standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 156pg/mL-10,000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- 抗原 See all SLAMF1 ELISA试剂盒
- SLAMF1 (Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1))
- 别名
- SLAM - CD150 (SLAMF1 产品)
- 别名
- CD150 ELISA Kit, SLAM ELISA Kit, SLAMF1 ELISA Kit, 4933415F16 ELISA Kit, AA177906 ELISA Kit, CDw150 ELISA Kit, ESTM51 ELISA Kit, IPO-3 ELISA Kit, Slam ELISA Kit, RGD1560634 ELISA Kit, signaling lymphocytic activation molecule family member 1 ELISA Kit, SLAMF1 ELISA Kit, Slamf1 ELISA Kit
- 基因ID
- 6504
- NCBI登录号
- NP_003028
- UniProt
- Q13291
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