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Thrombospondin 1 ELISA 试剂盒

Rockland koa0540 THBS1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
产品编号 ABIN6720022
Supplier Product No.: koa0540
发货至: 中国
  • 抗原 See all Thrombospondin 1 (THBS1) ELISA试剂盒
    Thrombospondin 1 (THBS1)
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    1.56 ng/mL - 100 ng/mL
    最低检测浓度
    1.56 ng/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0540
    Supplier
    Rockland
    原理
    Human THBS1 - TSP1 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant human THBS1. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 50 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product THBS1 ELISA Kit
  • 应用备注

    ELISA_Dilution: 1.56 ng/mL-100 ng/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human THBS1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human THBS1 standard solution and each sample is measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: N19-P1170

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all Thrombospondin 1 (THBS1) ELISA试剂盒
    Thrombospondin 1 (THBS1)
    别名
    THBS1/TSP1 (THBS1 产品)
    背景

    Synonyms: THBS 1, THBS, THBS1, Thrombospondin 1, Thrombospondin-1, Tsp 1, TSP, Tsp1, TSP1_HUMAN

    Background: Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.

    基因ID
    7057
    NCBI登录号
    NP_003237
    UniProt
    P07996
    途径
    Autophagy
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