Thrombospondin 1 ELISA 试剂盒
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- 抗原 See all Thrombospondin 1 (THBS1) ELISA试剂盒
- Thrombospondin 1 (THBS1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0540
- Supplier
- Rockland
- 原理
- Human THBS1 - TSP1 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human THBS1. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 50 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product THBS1 ELISA Kit
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- 应用备注
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ELISA_Dilution: 1.56 ng/mL-100 ng/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human THBS1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human THBS1 standard solution and each sample is measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: N19-P1170
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all Thrombospondin 1 (THBS1) ELISA试剂盒
- Thrombospondin 1 (THBS1)
- 别名
- THBS1/TSP1 (THBS1 产品)
- 背景
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Synonyms: THBS 1, THBS, THBS1, Thrombospondin 1, Thrombospondin-1, Tsp 1, TSP, Tsp1, TSP1_HUMAN
Background: Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.
- 基因ID
- 7057
- NCBI登录号
- NP_003237
- UniProt
- P07996
- 途径
- Autophagy
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