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IL-6 ELISA 试剂盒

Rockland koa0227 IL6 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Rockland
产品编号 ABIN6720002
Supplier Product No.: koa0227
发货至: 中国
  • 抗原 See all IL-6 (IL6) ELISA试剂盒
    IL-6 (IL6) (Interleukin 6 (IL6))
    适用
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    62.5 pg/mL - 4000 pg/mL
    最低检测浓度
    62.5 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0227
    Supplier
    Rockland
    原理
    Rat IL-6 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant rat IL-6. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 5 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product IL6 ELISA Kit
  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat IL-6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each rat IL-6 standard solution and each sample be measured in duplicate.
    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: F25-T211

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all IL-6 (IL6) ELISA试剂盒
    IL-6 (IL6) (Interleukin 6 (IL6))
    别名
    IL-6 (IL6 产品)
    背景

    Synonyms: B cell differentiation factor, B cell stimulatory factor 2, B-cell stimulatory factor 2, BSF 2, BSF-2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating fact, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFN-beta-2, IFNB2, IL 6, IL-6, IL6, IL6 protein, IL6_HUMAN, Interferon beta 2, Interferon beta-2, Interleukin 6, Interleukin 6 (interferon beta 2), Interleukin BSF 2, Interleukin-6, Interleukin6

    Background: Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6(IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone(CRH). Interleukin-6(IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor(IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8 kDa.

    基因ID
    24498
    NCBI登录号
    NP_036721
    UniProt
    P20607
    途径
    TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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