VEGF ELISA 试剂盒
-
- 抗原 See all VEGF ELISA试剂盒
- VEGF (Vascular Endothelial Growth Factor (VEGF))
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0331
- Supplier
- Rockland
- 原理
- Mouse VEGF Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
- Analytical Method
- Quantitative
- 特异性
-
Production: This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 2 pg/mL
- 组件
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product VEGF ELISA Kit
-
Vascular Endothelial Growth Factor (VEGF) ELISA Kit
VEGF 适用: 人 AA 27-191 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 大鼠 AA 27-190 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 小鼠 AA 27-190 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Vascular Endothelial Growth Factor (VEGF) IQ-ELISA KitVEGF 适用: 人 qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 兔 Colorimetric Sandwich ELISA 1.563 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 犬 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
-
- 应用备注
-
ELISA_Dilution: 15.6pg/mL-1000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each mouse VEGF standard solution and each sample is measured in duplicate.
- 说明
-
Standard: Expression system for standard: sf21, Immunogen sequence: A27-R190
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
-
- 抗原 See all VEGF ELISA试剂盒
- VEGF (Vascular Endothelial Growth Factor (VEGF))
- 别名
- VEGF (VEGF 产品)
- 背景
-
Synonyms: MGC70609, MVCD1, Vascular endothelial growth factor, Vascular endothelial growth factor A, Vascular permeability factor, VEGF A, VEGFA, VPF
Background: Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.
- 基因ID
- 22339
- NCBI登录号
- NP_001020421
- UniProt
- Q00731
-
