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PLAU ELISA 试剂盒

PLAU 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
产品编号 ABIN6719975
发货至: 中国
  • 抗原 See all PLAU ELISA试剂盒
    PLAU (Plasminogen Activator, Urokinase (PLAU))
    适用
    • 12
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    62.5 pg/mL - 4000 pg/mL
    最低检测浓度
    62.5 pg/mL
    应用范围
    ELISA
    原理
    Sandwich ELISA for Quantitative Detection of Antigen
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    产品特性

    Synonyms: ATF, BDPLT5, Plasminogen activator urinary, PLAU, QPD, u PA, U-plasminogen activator, uPA, URK, UROK_HUMAN, Urokinase-type plasminogen activator chain B

    Background: Plasminogen activator,urokinase(PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease(AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell(VSMC), uPA stimulates migration via the uPA receptor(uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase(PI3-K).

    Gene Name: PLAU

    Production: Natural and recombinant human Upa. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: M1-L431

    Top Product
    Discover our top product PLAU ELISA Kit
  • 应用备注

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human uPA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human uPA standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 62.5pg/mL-4000pg/mL

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • 抗原 See all PLAU ELISA试剂盒
    PLAU (Plasminogen Activator, Urokinase (PLAU))
    别名
    uPA - PLAU (PLAU 产品)
    别名
    PLAU ELISA Kit, u-PA ELISA Kit, ATF ELISA Kit, BDPLT5 ELISA Kit, QPD ELISA Kit, UPA ELISA Kit, URK ELISA Kit, uPA ELISA Kit, UPAM ELISA Kit, plasminogen activator, urokinase ELISA Kit, urokinase-type plasminogen activator ELISA Kit, PLAU ELISA Kit, CpipJ_CPIJ002131 ELISA Kit, CpipJ_CPIJ006543 ELISA Kit, CpipJ_CPIJ013396 ELISA Kit, CpipJ_CPIJ013623 ELISA Kit, Plau ELISA Kit
    基因ID
    5328
    NCBI登录号
    NP_001138503
    UniProt
    P00749
    途径
    Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
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