FAP ELISA 试剂盒
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- 抗原 See all FAP ELISA试剂盒
- FAP (Fibroblast Activation Protein, alpha (FAP))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5 pg/mL - 4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0627
- Supplier
- Rockland
- 原理
- Human Seprase - FAP Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human Seprase. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product FAP ELISA Kit
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Fibroblast Activation Protein, alpha (FAP) ELISA Kit
FAP 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 人 AA 26-760 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Plasma, Serum
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 人 Colorimetric Sandwich ELISA 12-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 人 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
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ELISA_Dilution: 62.5pg/mL-4000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Seprase standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Seprase standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: L26-D760
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all FAP ELISA试剂盒
- FAP (Fibroblast Activation Protein, alpha (FAP))
- 别名
- Seprase/FAP (FAP 产品)
- 背景
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Synonyms: 170 kDa melanoma membrane-bound gelatinase, FAP, Fibroblast activation protein alpha, Integral membrane serine protease, SEPR_HUMAN, Seprase
Background: FAP(Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50 % of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position. FAP-alpha shows 48 % amino acid identity with dipeptidyl peptidase IV and 30 % identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2 % of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.
- 基因ID
- 2191
- NCBI登录号
- NP_001278736
- UniProt
- Q12884
- 途径
- Tube Formation
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