Soluble Vascular Endothelial Growth Factor Receptor 1(sFlt-1/sVEGFR-1) ELISA 试剂盒
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- 抗原
- Soluble Vascular Endothelial Growth Factor Receptor 1(sFlt-1/sVEGFR-1)
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0332
- Supplier
- Rockland
- 原理
- Human sVEGFR1 - sFLT1 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human Svegfr1. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 30 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Soluble Vascular Endothelial Growth Factor Receptor 1(sFlt-1/sVEGFR-1) ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
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ELISA_Dilution: 156pg/mL-10000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human sVEGFR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each human sVEGFR1 standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: sf21, Immunogen sequence: S27-H687
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- Soluble Vascular Endothelial Growth Factor Receptor 1(sFlt-1/sVEGFR-1)
- 别名
- sVEGFR1/sFLT1
- 背景
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Synonyms: FLT, FLT 1, Flt-1, FLT1, Fms like tyrosine kinase 1, Fms related tyrosine kinase 1, Fms-like tyrosine kinase 1, Tyrosine protein kinase FRT, Tyrosine protein kinase receptor FLT, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular endothelial growth factor receptor 1, Vascular endothelial growth factor/vascular permeability factor receptor, Vascular permeability factor receptor, VEGFR 1, VEGFR-1, VEGFR1, VGFR1_HUMAN
Background: sVEGFR1, also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.
- 基因ID
- 2321
- NCBI登录号
- NP_001153392
- UniProt
- P17948
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