IL-7 ELISA 试剂盒
-
- 抗原 See all IL-7 (IL7) ELISA试剂盒
- IL-7 (IL7) (Interleukin 7 (IL7))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0459
- Supplier
- Rockland
- 原理
- Human IL-7 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA), Serum
- Analytical Method
- Quantitative
- 特异性
-
Production: Natural and recombinant human IL-7. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 1 pg/mL
- 组件
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product IL7 ELISA Kit
-
-
- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each human IL-7 standard solution and each sample be measured in duplicate.
- 说明
-
Standard: Expression system for standard: E.coli, Immunogen sequence: D26-H177
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
-
- 抗原 See all IL-7 (IL7) ELISA试剂盒
- IL-7 (IL7) (Interleukin 7 (IL7))
- 别名
- IL-7 (IL7 产品)
- 背景
-
Synonyms: IL 7, IL7 nirs variant 6, Interleukin 7, Interleukin7
Background: IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7(IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor(HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta(TCRB) during early T cell development.
- 基因ID
- 3574
- NCBI登录号
- NP_000871
- UniProt
- A8K673
- 途径
- JAK/STAT Signaling
-