CD25 ELISA 试剂盒
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- 抗原 See all CD25 (IL2RA) ELISA试剂盒
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0552
- Supplier
- Rockland
- 原理
- Mouse CD25 - IL-2sR alpha Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant mouse CD25. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 5 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product IL2RA ELISA Kit
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Interleukin 2 Receptor, alpha (IL2RA) ELISA Kit
IL2RA 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 小鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: Cow Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 人 Colorimetric Sandwich ELISA 15-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 人 AA 22-213 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 小鼠 AA 22-236 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 人 Soluble Colorimetric Sandwich ELISA 78.13-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 人 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse CD25 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. We recommend that each mouse CD25 standard solution and each sample is measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: E22-K236
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all CD25 (IL2RA) ELISA试剂盒
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- 别名
- CD25/IL-2sR alpha (IL2RA 产品)
- 背景
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Synonyms: CD122, CD122 antigen, CD132, CD132 antigen, CD25, CD25 antigen, Cytokine receptor common gamma chain, Gamma C, High affinity IL 2 receptor subunit beta, IL 2 RA, IL 2 receptor, IL 2 receptor alpha subunit, IL 2R gamma chain, IL2R, IL2RA, IL2RB, IL2RG, IMD4, interleukin 2 receptor alpha, Interleukin 2 receptor alpha chain, interleukin 2 receptor beta, Interleukin 2 receptor beta chain, interleukin 2 receptor gamma, Interleukin 2 receptor gamma chain, p55, p64, P70 75, p75, SCIDX, SCIDX1, TAC antigen, TCGFR, XSCID
Background: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
- 基因ID
- 16184
- NCBI登录号
- NP_032393
- UniProt
- P01590
- 途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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