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CD44 ELISA 试剂盒

Rockland koa0857 CD44 适用: 小鼠 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
产品编号 ABIN6719921
Supplier Product No.: koa0857
发货至: 中国
  • 抗原 See all CD44 ELISA试剂盒
    CD44
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    312 pg/mL - 20000 pg/mL
    最低检测浓度
    312 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0857
    Supplier
    Rockland
    原理
    Mouse CD44 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant mouse CD44. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 10 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse CD44 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD44 standard solution and each sample be measured in duplicate.
    说明

    Standard: Expression system for standard: CHO, Immunogen sequence: Q25-T224

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all CD44 ELISA试剂盒
    CD44
    别名
    CD44 (CD44 产品)
    背景

    Synonyms: LHR, BA-1, CD44 antigen, CD44 Molecule, CD44_HUMAN, CDw44, Cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HCELL, hematopoietic cell E- and L-selectin ligand, Heparan sulfate proteoglycan, Hermes antigen, homing function and Indian blood group system, HSA, HUTCH-I, HUTCH1, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH1, PGP1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

    Background: CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

    基因ID
    12505
    NCBI登录号
    NP_001034240
    UniProt
    P15379
    途径
    Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
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