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IL-6 ELISA 试剂盒

Rockland koa0225 IL6 适用: 人 Colorimetric Sandwich ELISA 4.69 pg/mL - 300 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Rockland
产品编号 ABIN6719918
Supplier Product No.: koa0225
发货至: 中国
  • 抗原 See all IL-6 (IL6) ELISA试剂盒
    IL-6 (IL6) (Interleukin 6 (IL6))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    4.69 pg/mL - 300 pg/mL
    最低检测浓度
    4.69 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0225
    Supplier
    Rockland
    原理
    Human IL-6 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant human IL-6 . There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 0.3 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product IL6 ELISA Kit
  • 应用备注

    ELISA_Dilution: 4.69pg/mL-300pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.75pg/mL, 9.38pg/mL, 4.69pg/mL human IL-6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. We recommend that each human IL-6 standard solution and each sample is measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: P29-M212

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all IL-6 (IL6) ELISA试剂盒
    IL-6 (IL6) (Interleukin 6 (IL6))
    别名
    IL-6 (IL6 产品)
    背景

    Synonyms: B cell differentiation factor, B cell stimulatory factor 2, B-cell stimulatory factor 2, BSF 2, BSF-2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating fact, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFN-beta-2, IFNB2, IL 6, IL-6, IL6, IL6 protein, IL6_HUMAN, Interferon beta 2, Interferon beta-2, Interleukin 6, Interleukin 6 (interferon beta 2), Interleukin BSF 2, Interleukin-6, Interleukin6

    Background: The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3 kDa.

    基因ID
    3569
    NCBI登录号
    NP_000591
    UniProt
    B4DVM1
    途径
    TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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