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IL-2 ELISA 试剂盒

Rockland koa0214 IL2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Rockland
产品编号 ABIN6719915
Supplier Product No.: koa0214
发货至: 中国
  • 抗原 See all IL-2 (IL2) ELISA试剂盒
    IL-2 (IL2) (Interleukin 2 (IL2))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.6 pg/mL - 1000 pg/mL
    最低检测浓度
    15.6 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0214
    Supplier
    Rockland
    原理
    Human IL-2 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant human IL-2. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 1 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product IL2 ELISA Kit
  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. It is recommended that each human IL-2 standard solution and each sample be measured in duplicate.
    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: A21-T153

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all IL-2 (IL2) ELISA试剂盒
    IL-2 (IL2) (Interleukin 2 (IL2))
    别名
    IL-2 (IL2 产品)
    背景

    Synonyms: Aldesleukin, IL 2, IL-2, IL2, IL2_HUMAN, Interleukin 2, Interleukin-2, Interleukin2, Involved in regulation of T cell clonal expansion, Lymphokine, T cell growth factor, T-cell growth factor, TCGF

    Background: Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.

    基因ID
    3558
    NCBI登录号
    NP_000577
    UniProt
    P60568
    途径
    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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