Osteoactivin ELISA 试剂盒
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- 抗原 See all Osteoactivin (GPNMB) ELISA试剂盒
- Osteoactivin (GPNMB)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: Gpnmb, GPNMB_HUMAN, Transmembrane glycoprotein HGFIN, Transmembrane glycoprotein NMB
Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis(soft callus) and osteogenesis(hard callus) compared to the intact femurs that is why Osteoactivin(OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.
Gene Name: GPNMB
Production: Natural and recombinant human GPNMB. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: A22-P486
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Osteoactivin (GPNMB) ELISA Kit
GPNMB 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 AA 22-486 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 小鼠 AA 23-502 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 小鼠 Colorimetric Sandwich ELISA 80-20.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 Colorimetric Sandwich ELISA 45-12.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 Colorimetric Sandwich ELISA Cell Culture Cells, Plasma, Serum
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human GPNMB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human GPNMB standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 156pg/mL-10,000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all Osteoactivin (GPNMB) ELISA试剂盒
- Osteoactivin (GPNMB)
- 别名
- GPNMB - Osteoactivin (GPNMB 产品)
- 别名
- HGFIN ELISA Kit, NMB ELISA Kit, DC-HIL ELISA Kit, Dchil ELISA Kit, ipd ELISA Kit, glycoprotein nmb ELISA Kit, glycoprotein (transmembrane) nmb ELISA Kit, glycoprotein nmb L homeolog ELISA Kit, Gpnmb ELISA Kit, GPNMB ELISA Kit, gpnmb.L ELISA Kit
- 基因ID
- 10457
- NCBI登录号
- NP_001005340
- UniProt
- Q14956
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