ERBB3 ELISA 试剂盒
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- 抗原 See all ERBB3 ELISA试剂盒
- ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: c erbB3, Erb b2 receptor tyrosine kinase 3, ERBB3 protein, erbB3 S, ERBB3_HUMAN, Glial growth factor receptor, HER 3, R3, Human epidermal growth factor receptor 3, LCCS2, MDA BF 1, p180 ErbB3, p45 sErbB3, p85 sErbB3, proto-oncogene-like protein c ErbB3, Receptor tyrosine protein kinase erbB 3, Tyrosine kinase type cell surface receptor HER3, Tyrosine kinase-type cell surface receptor HER3, v erb b2 avian erythroblastic leukemia viral oncogene homolog 3, v erb b2 erythroblastic leukemia viral oncogene homolog 3
Background: Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene. ErbB3 has been shown to bind the ligands heregulin and NRG-2. Ligand binding causes a change in conformation that allows for dimerization, phosphorylation, and activation of signal transduction. ErbB3 can heterodimerize with any of the other three ErbB family members. The theoretical ErbB3 homodimer would be non-functional because the kinase-impaired protein requires transphosporylation by its binding partner to be active. Unlike the other ErbB receptor tyrosine kinase family members which are activated through autophosphorylation upon ligand binding, ErbB3 is found to be kinase impaired, having only 1/1000th the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins. Therefore, ErbB3 must act as anallosteric activator.
Gene Name: ERBB3
Production: Natural and recombinant human ERBB3. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: S20-T643
- Top Product
- Discover our top product ERBB3 ELISA Kit
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Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) ELISA Kit
ERBB3 适用: 人 pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) ELISA KitERBB3 适用: 人 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) ELISA KitERBB3 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum
Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) ELISA KitERBB3 适用: 人 AA 20-643 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human ERBB3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ERBB3 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 156pg/mL-10,000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all ERBB3 ELISA试剂盒
- ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))
- 别名
- ERBB3 - Her3 (ERBB3 产品)
- 别名
- ErbB-3 ELISA Kit, HER3 ELISA Kit, LCCS2 ELISA Kit, MDA-BF-1 ELISA Kit, c-erbB-3 ELISA Kit, c-erbB3 ELISA Kit, erbB3-S ELISA Kit, p180-ErbB3 ELISA Kit, p45-sErbB3 ELISA Kit, p85-sErbB3 ELISA Kit, ERBB3 ELISA Kit, C76256 ELISA Kit, Erbb-3 ELISA Kit, Erbb3r ELISA Kit, Her3 ELISA Kit, nuc-ErbB3 ELISA Kit, erb-b2 receptor tyrosine kinase 3 ELISA Kit, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) ELISA Kit, ERBB3 ELISA Kit, Erbb3 ELISA Kit
- 基因ID
- 2065
- NCBI登录号
- NP_001005915
- UniProt
- P21860
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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