电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

Nidogen 1 ELISA 试剂盒

Rockland koa0517 NID1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
Rockland
产品编号 ABIN6719872
Supplier Product No.: koa0517
发货至: 中国
  • 抗原 See all Nidogen 1 (NID1) ELISA试剂盒
    Nidogen 1 (NID1)
    适用
    • 5
    • 2
    • 2
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    62.5 pg/mL - 4000 pg/mL
    最低检测浓度
    62.5 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0517
    Supplier
    Rockland
    原理
    Human Entactin - NID-1 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant human Nidogen-1. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 10 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Featured
    Discover our best selling NID1 ELISA Kit
    Top Product
    Discover our top product NID1 ELISA Kit
  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Nidogen-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human Nidogen-1 standard solution and each sample be measured in duplicate.
    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: L29-K1114

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all Nidogen 1 (NID1) ELISA试剂盒
    Nidogen 1 (NID1)
    别名
    Nidogen-1/Entactin/NID-1 (NID1 产品)
    背景

    Synonyms: Entactin, NID-1, Nid1, NID1_HUMAN, Nidogen-1

    Background: Nidogen-1(NID-1), also known as Entactin, is a protein that in humans is encoded by the NID1 gene. It is a member of the nidogen family of basement membrane glycoproteins. This gene is mapped to 1q42.3. Nidogen-1 is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans(heparan sulfate and glycosaminoglycans), laminin and fibronectin. The protein interacts with several other components of basement membranes. Structurally it(along with perlecan) connects the networks formed by collagens and laminins to each other. It may also play a role in cell interactions with the extracellular matrix. Nidogen-1 also can serve as a bridge between the 2 most abundant molecules in the basement membrane: type IV collagen and laminin.

    基因ID
    4811
    NCBI登录号
    NP_002499
    UniProt
    P14543
You are here:
客服