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CCL18 ELISA 试剂盒

Rockland koa0396 CCL18 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Serum
Rockland
产品编号 ABIN6719861
Supplier Product No.: koa0396
发货至: 中国
  • 抗原 See all CCL18 ELISA试剂盒
    CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    7.8 pg/mL - 500 pg/mL
    最低检测浓度
    7.8 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0396
    Supplier
    Rockland
    原理
    Human CCL18 - PARC Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant human CCL18. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 10 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human CCL18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. We recommend that each human CCL18 standard solution and each sample is measured in duplicate.
    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: A21-A89

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all CCL18 ELISA试剂盒
    CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
    别名
    CCL18/PARC (CCL18 产品)
    背景

    Synonyms: Alternative macrophage activation associated CC chemokine 1, Alternative macrophage activation-associated CC chemokine 1, AMAC1, C C motif chemokine 18, CC chemokine ligand 18, CC chemokine PARC, CCL18_HUMAN, Chemokine ligand 18, Chemokine dendritic, Chemokine ligand 18, CKb7, DCCK1, Dendritic cell chemokine 1, Macrophage inflammatory protein 4, MIP4, PARC, Pulmonary and activation regulated chemokine, Pulmonary and activation-regulated chemokine, Small inducible cytokine A18

    Background: Chemokine(C-C motif) ligand 18(CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC(pulmonary and activation-regulated chemokine). CCL18 is approximately 60 % identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.

    基因ID
    6362
    NCBI登录号
    NP_002979
    UniProt
    P55774
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