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IL-5 ELISA 试剂盒

IL5 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Serum
产品编号 ABIN6719837
发货至: 中国
  • 抗原 See all IL-5 (IL5) ELISA试剂盒
    IL-5 (IL5) (Interleukin 5 (IL5))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.6 pg/mL - 1000 pg/mL
    最低检测浓度
    15.6 pg/mL
    应用范围
    ELISA
    原理
    Sandwich ELISA for Quantitative Detection of Antigen
    样品类型
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    产品特性

    Synonyms: B cell differentiation factor I, B-cell differentiation factor I, Colony stimulating factor eosinophil, EDF, Eosinophil differentiation factor, IL 5, IL-5, IL5, IL5_HUMAN, Interleukin 5, Interleukin 5 (colony stimulating factor eosinophil), Interleukin-5 Interleukin5, T cell replacing factor, T-cell replacing factor, TRF

    Background: Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70 % amino acid sequence homology.

    Gene Name: IL5

    Production: Natural and recombinant mouse IL-5. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: SF21, Immunogen sequence: M21-G133

    Top Product
    Discover our top product IL5 ELISA Kit
  • 应用备注

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse IL-5 standard solution and each sample be measured in duplicate.

    ELISA Dilution: 15.6pg/mL-1000pg/mL

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • 抗原 See all IL-5 (IL5) ELISA试剂盒
    IL-5 (IL5) (Interleukin 5 (IL5))
    别名
    IL-5 (IL5 产品)
    基因ID
    16191
    NCBI登录号
    NP_034688
    UniProt
    P04401
    途径
    JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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