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PD-1 ELISA 试剂盒

Rockland koa0589 PDCD1 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant
Rockland
产品编号 ABIN6719831
Supplier Product No.: koa0589
发货至: 中国
  • 抗原 See all PD-1 (PDCD1) ELISA试剂盒
    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
    适用
    • 7
    • 2
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    156 pg/mL - 10000 pg/mL
    最低检测浓度
    156 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0589
    Supplier
    Rockland
    原理
    Human PD-1 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant human PD-1. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 10 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product PDCD1 ELISA Kit
  • 应用备注

    ELISA_Dilution: 156pg/mL-10000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human PD-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates to each empty well. It is recommended that each human PD-1 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: L25-Q167

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all PD-1 (PDCD1) ELISA试剂盒
    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
    别名
    PD-1 (PDCD1 产品)
    背景

    Synonyms: CD279, CD279 antigen, hPD 1, hPD-1, PD 1, PD-1, PD1, PDCD 1, PDCD1, PDCD1_HUMAN, Programmed cell death 1, Programmed cell death protein 1, Protein PD 1, Protein PD-1, SLEB2

    Background: Programmed cell death protein 1, also called PD-1 or PDCD1 is a protein that in humans is encoded by the PDCD1 gene. This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. By fluorescence in situ hybridization, the PDCD1 gene is mapped to 2q37.3. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.

    基因ID
    5133
    NCBI登录号
    NP_005009
    UniProt
    Q15116
    途径
    Cancer Immune Checkpoints
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