NGFB ELISA 试剂盒
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- 抗原 See all NGFB ELISA试剂盒
- NGFB (Nerve Growth Factor beta (NGFB))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0278
- Supplier
- Rockland
- 原理
- Rat NGF - NGF beta Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant rat NGF. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 1 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Nerve Growth Factor beta (NGFB) ELISA Kit
NGFB 适用: 人 Colorimetric Sandwich ELISA 6.86 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB 适用: 人 Colorimetric Sandwich ELISA 14-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nerve Growth Factor beta (NGFB) ELISA KitNGFB 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB 适用: 大鼠 Colorimetric Sandwich ELISA 39.063 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB 适用: 兔 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB 适用: 大鼠 Colorimetric Sandwich ELISA 15-15000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat NGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants or serum to each empty well. It is recommended that each rat NGF standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: S122-G241
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all NGFB ELISA试剂盒
- NGFB (Nerve Growth Factor beta (NGFB))
- 别名
- NGF/NGF beta (NGFB 产品)
- 背景
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Synonyms: Beta nerve growth factor, Beta NGF, HSAN5, MGC161426, MGC161428, Nerve growth factor (beta polypeptide), Nerve growth factor beta polypeptide, Nerve growth factor beta subunit, NGFB, NID69
Background: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.
- 基因ID
- 310738
- NCBI登录号
- NP_001263984
- UniProt
- P25427
- 途径
- NF-kappaB Signaling, RTK signaling, Regulation of Cell Size
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