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M-CSF/CSF1 ELISA 试剂盒

Rockland koa0254 CSF1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum, Tissue Lysates
Rockland
产品编号 ABIN6719805
Supplier Product No.: koa0254
发货至: 中国
  • 抗原 See all M-CSF/CSF1 (CSF1) ELISA试剂盒
    M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
    适用
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    • 11
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.6 pg/mL - 1000 pg/mL
    最低检测浓度
    15.6 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0254
    Supplier
    Rockland
    原理
    Mouse M-CSF Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA), Serum, Tissue Lysates
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant mouse M-CSF. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 1 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product CSF1 ELISA Kit
  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse M-CSF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, tissue lysates, serum or plasma (EDTA) to each empty well. It is recommended that each mouse M-CSF standard solution and each sample be measured in duplicate.
    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence:  K33-E262

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all M-CSF/CSF1 (CSF1) ELISA试剂盒
    M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
    别名
    M-CSF (CSF1 产品)
    背景

    Synonyms: Colony stimulating factor 1, Colony stimulating factor 1 (macrophage), Colony stimulating factor macrophage specific, CSF 1, CSF-1, CSF1, CSF1_HUMAN, Csfm, Lanimostim, M CSF, M-CSF, Macrophage colony stimulating factor, Macrophage colony stimulating factor 1, MCSF, MGC41930, op, OTTHUMP00000013364, OTTHUMP00000013365, OTTMUSP00000032803, OTTMUSP00000032804, Processed macrophage colony-stimulating factor 1, RP11-195M16.2

    Background: M-CSF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb1. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb2. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing3. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines4.

    基因ID
    12977
    NCBI登录号
    NP_001107001
    UniProt
    P07141
    途径
    RTK signaling
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