TIMP3 ELISA 试剂盒
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- 抗原 See all TIMP3 ELISA试剂盒
- TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0315
- Supplier
- Rockland
- 原理
- Human TIMP-3 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human TIMP-3. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 2 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product TIMP3 ELISA Kit
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- 应用备注
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ELISA_Dilution: 156pg/mL-10000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TIMP-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin , EDTA) or saliva to each empty well. It is recommended that each human TIMP-3 standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: C24-P211
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all TIMP3 ELISA试剂盒
- TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))
- 别名
- TIMP-3 (TIMP3 产品)
- 背景
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Synonyms: HSMRK222, K222, K222TA2, Metalloproteinase inhibitor 3, MIG 5 protein, Protein MIG-5, SFD, TIMP metallopeptidase inhibitor 3, TIMP-3, TIMP3, TIMP3_HUMAN, Tissue inhibitor of metalloproteinases 3
Background: The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).
- 基因ID
- 7078
- NCBI登录号
- NP_000353
- UniProt
- P35625
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