PLGF ELISA 试剂盒
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- 抗原 See all PLGF (PGF) ELISA试剂盒
- PLGF (PGF) (Placenta Growth Factor (PGF))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0292
- Supplier
- Rockland
- 原理
- Human PLGF Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human PLGF. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 1 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product PGF ELISA Kit
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Placenta Growth Factor (PGF) ELISA Kit
PGF 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 大鼠 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 大鼠 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
Placenta Growth Factor (PGF) ELISA KitPGF 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/M - 1000 pg/M Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 人 AA 21-149 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 小鼠 Colorimetric Sandwich ELISA 1-400 pg/mL Cell Culture Supernatant, Plasma, Serum
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 小鼠 AA 24-158 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
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ELISA_Dilution: 15.6pg/mL-1000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human PLGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human PLGF standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: A21-R149
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all PLGF (PGF) ELISA试剂盒
- PLGF (PGF) (Placenta Growth Factor (PGF))
- 别名
- PLGF (PGF 产品)
- 背景
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Synonyms: AI854365, D12S1900, MGC93298, Pgf, PGFL, PIGF, Placenta growth factor, Placental growth factor, Placental growth factor vascular endothelial growth factor related protein, PlGF, PlGF 2, PLGF_HUMAN, PlGF2, SHGC 10760, Vascular endothelial growth factor related protein
Background: The protein, also called PGF, is 149 amino acids long and shares 53 % identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
- 基因ID
- 5228
- NCBI登录号
- NP_001193941
- UniProt
- P49763
- 途径
- VEGFR1 Specific Signals
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