CXCL10 ELISA 试剂盒
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- 抗原 See all CXCL10 ELISA试剂盒
- CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0429
- Supplier
- Rockland
- 原理
- Human CXCL10 - IP-10 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human CXCL10. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 1 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product CXCL10 ELISA Kit
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- 应用备注
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ELISA_Dilution: 31.2pg/mL-2000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CXCL10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA), saliva or urine to each empty well. It is recommended that each human CXCL10 standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: V22-P98
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all CXCL10 ELISA试剂盒
- CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
- 别名
- CXCL10/IP-10 (CXCL10 产品)
- 背景
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Synonyms: 10 kDa interferon gamma-induced protein, C7, Chemokine CXC motif ligand 10, Crg 2, CRG2, CXCL10, CXL10_HUMAN, Gamma IP10, gIP 10, GIP10, IFI10, INP 10, Interferon activated gene 10, Interferon gamma induced cell line, Interferon inducible cytokine IP10, IP 10, IP-10, MOB1, SCYB10, Small inducible cytokine B10, Small inducible cytokine B10 precursor, Small inducible cytokine subfamily B (Cys X Cys) member 10, Small inducible cytokine subfamily B CXC member 10, Small-inducible cytokine B10
Background: Chemokine(C-X-C motif) ligand 10(CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein(gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.
- 基因ID
- 3627
- NCBI登录号
- NP_001556
- UniProt
- P02778
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