VEGFB ELISA 试剂盒
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- 抗原 See all VEGFB ELISA试剂盒
- VEGFB (Vascular Endothelial Growth Factor B (VEGFB))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0852
- Supplier
- Rockland
- 原理
- Mouse VEGF-B Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant mouse VEGF-B. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 2 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product VEGFB ELISA Kit
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- 应用备注
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ELISA_Dilution: 31.2pg/mL-2000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse VEGF-B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each mouse VEGF-B standard solution and each sample is measured in duplicate.
- 说明
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Standard: Expression system for standard: sf21, Immunogen sequence: Q20-A207&Q20-R148
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all VEGFB ELISA试剂盒
- VEGFB (Vascular Endothelial Growth Factor B (VEGFB))
- 别名
- VEGF-B (VEGFB 产品)
- 背景
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Synonyms: Vascular endothelial growth factor B, VEGF B, VEGF related factor, VEGF-B, VEGF-related factor, Vegfb, VEGFB_HUMAN, VEGFL, VRF
Background: Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.
- 基因ID
- 22340
- NCBI登录号
- NP_001172093
- UniProt
- P49766
- 途径
- RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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