EpCAM ELISA 试剂盒
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- 抗原 See all EpCAM (EPCAM) ELISA试剂盒
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 93.7 pg/mL - 6000 pg/mL
- 最低检测浓度
- 93.7 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0446
- Supplier
- Rockland
- 原理
- Human EPCAM Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human EPCAM. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product EPCAM ELISA Kit
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/m human EPCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human EPCAM standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: Q24-K265
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all EpCAM (EPCAM) ELISA试剂盒
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- 别名
- EPCAM (EPCAM 产品)
- 背景
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Synonyms: 17 1A, 323/A3, AUA1, CD326, CD326 antigen, CO 17A, CO17 1A, CO17A, DIAR5, EGP2, EGP, EGP2, EGP314, EGP40, Ep CAM, Ep-CAM, EPCAM, ESA, GA733 1, GA733 2, GA733-2, hEGP 2, hEGP314, HNPCC8, KS1/4, KSA, M1S 1, M1S2 ,M4S1, MIC18 MK 1 ,TACD1, TACSTD1, TROP1
Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. Also it is involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since it is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.
- 基因ID
- 4072
- NCBI登录号
- NP_002345
- UniProt
- P16422
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