CCL21 ELISA 试剂盒
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- 抗原 See all CCL21 ELISA试剂盒
- CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0336
- Supplier
- Rockland
- 原理
- Mouse CCL21 - 6Ckine Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant mouse CCL21.There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product CCL21 ELISA Kit
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- 应用备注
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ELISA_Dilution: 15.6pg/mL-1000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CCL21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CCL21 standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: S24-G133
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all CCL21 ELISA试剂盒
- CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))
- 别名
- CCL21/6Ckine (CCL21 产品)
- 背景
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Synonyms: 6Ckine, Beta chemokine exodus 2, C-C motif chemokine 21, Chemokine (C-C motif) ligand 21, CKb9, ECL, Efficient Chemoattractant for Lymphocytes, Exodus2, MGC34555, OTTHUMP00000000526, OTTHUMP00000000527, OTTHUMP00000021303, SCYA 21, SCYA21, Secondary lymphoid tissue chemokine, SLC, Small inducible cytokine A21, Small inducible cytokine subfamily A (Cys-Cys), member 21, TCA 4, TCA4
Background: Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.
- 基因ID
- 18829
- NCBI登录号
- NP_001180596
- UniProt
- P84444
- 途径
- Regulation of Actin Filament Polymerization
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