alpha 2 Macroglobulin ELISA 试剂盒
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- 抗原 See all alpha 2 Macroglobulin (A2M) ELISA试剂盒
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 625 pg/mL - 40000 pg/mL
- 最低检测浓度
- 625 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0638
- Supplier
- Rockland
- 原理
- Human A2M-alpha2-Macroglobulin Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural human A2M. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 20 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product A2M ELISA Kit
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alpha-2-Macroglobulin (A2M) ELISA Kit
A2M 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 小鼠 Colorimetric Competition ELISA 3.7 ng/mL - 300 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitHigh Sensitivity A2M 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Competition ELISA 1.25 μg/mL - 20 μg/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Cell Culture Supernatant, Plasma, Serum
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Sandwich ELISA 78.125 ng/mL - 5000 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Sandwich ELISA 20 ng/mL - 640 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 大鼠 Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: Cow Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human A2M standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human A2M standard solution and each sample be measured in duplicate.
- 说明
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Standard: from human plasma
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all alpha 2 Macroglobulin (A2M) ELISA试剂盒
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
- 别名
- A2M/alpha2-Macroglobulin (A2M 产品)
- 背景
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Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, A2m, A2MD, A2MG_HUMAN, Alpha-2-M, Alpha-2-macroglobulin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, CPAMD5, FWP007, Pregnancy zone protein, S863-7
Background: Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease(AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates(activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
- 基因ID
- 2
- NCBI登录号
- NP_000005
- UniProt
- P01023
- 途径
- Lipid Metabolism
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