BDNF ELISA 试剂盒
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- 抗原 See all BDNF ELISA试剂盒
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: Abrineurin, ANON2, Brain derived neurotrophic factor, BULN2, MGC34632, Neurotrophin
Background: Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8 kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
Gene Name: BDNF
Production: Natural and recombinant human BDNF. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: sf21, Immunogen sequence: H129-R247
- Top Product
- Discover our top product BDNF ELISA Kit
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Brain-Derived Neurotrophic Factor (BDNF) ELISA Kit
BDNF 适用: 小鼠 AA 129-247 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 大鼠 AA 129-247 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 人, 大鼠, 小鼠, 兔, 马, 山羊 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 兔 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 豚鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 人 AA 129-247 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human BDNF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human BDNF standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 31.2pg/mL-2000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- 抗原 See all BDNF ELISA试剂盒
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
- 别名
- BDNF (BDNF 产品)
- 基因ID
- 627
- NCBI登录号
- NP_001137277
- UniProt
- P23560
- 途径
- RTK signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
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