Soluble E-Selectin ELISA 试剂盒
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- 抗原 See all Soluble E-Selectin (sE-Selectin) products
- Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0571
- Supplier
- Rockland
- 原理
- Rat sE-Selectin Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant rat sE-Selectin. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Selectin E (Soluble) (sE-Selectin) ELISA Kit
sE-Selectin 适用: 小鼠 AA 22-557 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (citrate), Plasma (heparin), Serum
Selectin E (Soluble) (sE-Selectin) ELISA KitsE-Selectin 适用: 大鼠 AA 22-494 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (citrate), Plasma (heparin), Serum
Selectin E (Soluble) (sE-Selectin) ELISA KitsE-Selectin 适用: 人 Colorimetric Sandwich ELISA 780-50000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Selectin E (Soluble) (sE-Selectin) ELISA KitsE-Selectin 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (heparin - citrate), Serum
Selectin E (Soluble) (sE-Selectin) ELISA KitsE-Selectin 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (heparin - citrate), Serum
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat E-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, citrate) to each empty well. It is recommended that each rat E-Selectin standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: W22-P494
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all Soluble E-Selectin (sE-Selectin) products
- Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))
- 别名
- sE-Selectin (sE-Selectin 产品)
- 背景
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Synonyms: CD62 antigen-like family member E, CD62E, CD62E antigen, E selectin, E-selectin, ELAM, ELAM-1, ELAM1, Endothelial leukocyte adhesion molecule 1, ESEL, LECAM2, Leukocyte endothelial cell adhesion molecule 2, Leukocyte-endothelial cell adhesion molecule 2, LYAM2_HUMAN, Sele
Background: E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM).3 A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4
- 基因ID
- 25544
- NCBI登录号
- NP_620234
- UniProt
- P98105
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