CCL9 ELISA 试剂盒
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- 抗原 See all CCL9 (Ccl9) ELISA试剂盒
- CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 7.8 pg/mL - 500 pg/mL
- 最低检测浓度
- 7.8 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0718
- Supplier
- Rockland
- 原理
- Mouse CCL9 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant mouse CCL9 . There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 1 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product Ccl9 ELISA Kit
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CCL9 standard solution and each sample is measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: Q22-Q122
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all CCL9 (Ccl9) ELISA试剂盒
- CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))
- 别名
- CCL9 (Ccl9 产品)
- 背景
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Synonyms: CCF18, CCL9, Chemokine CC motif ligand 9, Macrophage Inflammatory Protein 1 gamma, Macrophage inflammatory protein-related protein 2, MIP-1 gamma, mMIP1g, SCYA10, SCYA9, Small inducible cytokine A9
Background: Chemokine (C-C motif) ligand 9(CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium(FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1(the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.
- 基因ID
- 20308
- NCBI登录号
- NP_035468
- UniProt
- P51670
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