Thrombopoietin ELISA 试剂盒
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- 抗原 See all Thrombopoietin (THPO) ELISA试剂盒
- Thrombopoietin (THPO)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: C mpl ligand, Megakaryocyte colony stimulating factor, Megakaryocyte growth and development factor, Megakaryocyte stimulating factor, MGC164194, MGDF, MKCSF, ML, MPL ligand, MPLLG, Myeloproliferative leukemia virus oncogene ligand, OTTHUMP00000210504, THPO, TPO
Background: Thrombopoietin(TPO) is also called megakaryocyte growth and development factor(MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 419 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.
Gene Name: TPO
Production: Natural and recombinant mouse TPO. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: S22-T356
- Top Product
- Discover our top product THPO ELISA Kit
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse ell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse TPO standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 31.2pg/mL-2000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all Thrombopoietin (THPO) ELISA试剂盒
- Thrombopoietin (THPO)
- 别名
- Thrombopoietin (TPO) (THPO 产品)
- 别名
- MGDF ELISA Kit, MKCSF ELISA Kit, ML ELISA Kit, MPLLG ELISA Kit, THCYT1 ELISA Kit, TPO ELISA Kit, Mgdf ELISA Kit, Ml ELISA Kit, Mpllg ELISA Kit, Tpo ELISA Kit, Tpo1 ELISA Kit, Tpo2 ELISA Kit, Tpo3 ELISA Kit, Tpo4 ELISA Kit, tpo ELISA Kit, LOC100231762 ELISA Kit, thrombopoietin ELISA Kit, THPO ELISA Kit, Thpo ELISA Kit, thpo ELISA Kit
- 基因ID
- 21832
- NCBI登录号
- NP_001166976
- UniProt
- P40226
- 途径
- JAK/STAT Signaling, Hormone Activity
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