CD80 ELISA 试剂盒
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- 抗原 See all CD80 ELISA试剂盒
- CD80
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5 pg/mL - 4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: Activation B7-1 antigen, B7, BB1, CD80, CD80_HUMAN, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD82
Background: The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain.The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
Gene Name: CD80
Production: Natural and recombinant rat CD80. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: I39-Q248
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CD80 (CD80) ELISA Kit
CD80 适用: 人 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
CD80 (CD80) ELISA KitCD80 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
CD80 (CD80) ELISA KitCD80 适用: 小鼠 AA 37-245 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD80 (CD80) ELISA KitCD80 适用: 大鼠 AA 39-248 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD80 (CD80) ELISA KitCD80 适用: 人 AA 35-242 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD80 (CD80) ELISA KitCD80 适用: 小鼠 Colorimetric Sandwich ELISA 28-7.000 pg/mL Cell Culture Supernatant, Plasma, Serum
CD80 (CD80) ELISA KitCD80 适用: 人 Colorimetric Sandwich ELISA 12-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
CD80 (CD80) ELISA KitCD80 适用: 小鼠 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Plasma, Serum, Tissue Homogenate
CD80 (CD80) ELISA KitCD80 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat CD80 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. It is recommended that each rat CD80 standard solution and each sample be measured in duplicate.
ELISA Dilution: 62.5pg/mL-4000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- 抗原 See all CD80 ELISA试剂盒
- CD80
- 别名
- CD80 - B7-1 (CD80 产品)
- 别名
- B7 ELISA Kit, B7-1 ELISA Kit, B7.1 ELISA Kit, BB1 ELISA Kit, CD28LG ELISA Kit, CD28LG1 ELISA Kit, LAB7 ELISA Kit, B71 ELISA Kit, Cd28l ELISA Kit, Ly-53 ELISA Kit, Ly53 ELISA Kit, MIC17 ELISA Kit, TSA1 ELISA Kit, CD80 molecule ELISA Kit, Cd80 molecule ELISA Kit, CD80 antigen ELISA Kit, CD80 ELISA Kit, Cd80 ELISA Kit
- 基因ID
- 25408
- NCBI登录号
- NP_037058
- UniProt
- O35187
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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