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SDC4 ELISA 试剂盒

Rockland koa0819 SDC4 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
Rockland
产品编号 ABIN6719692
Supplier Product No.: koa0819
发货至: 中国
  • 抗原 See all SDC4 ELISA试剂盒
    SDC4 (Syndecan 4 (SDC4))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    62.5 pg/mL - 4000 pg/mL
    最低检测浓度
    62.5 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0819
    Supplier
    Rockland
    原理
    Human Syndecan-4 - SDC4 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant human Syndecan-4. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 10 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product SDC4 ELISA Kit
  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Syndecan-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human Syndecan-4 standard solution and each sample be measured in duplicate.
    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: E19-E145

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all SDC4 ELISA试剂盒
    SDC4 (Syndecan 4 (SDC4))
    别名
    Syndecan-4/SDC4 (SDC4 产品)
    背景

    Synonyms: Amphiglycan, MGC22217, OTTHUMP00000031788, ryudocan amphiglycan, Ryudocan, Ryudocan core protein, SDC 4, SDC4, SDC4_HUMAN, SYND 4, SYND4, syndecan 4, syndecan 4 (amphiglycan, ryudocan), syndecan proteoglycan 4, Syndecan-4, Syndecan4

    Background: Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What's more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.

    基因ID
    6385
    NCBI登录号
    NP_002990
    UniProt
    P31431
    途径
    Glycosaminoglycan Metabolic Process
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