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SPARCL1 ELISA 试剂盒

SPARCL1 适用: 人 Colorimetric Sandwich ELISA 46.9 pg/mL - 3000 pg/mL Cell Culture Supernatant, Serum
产品编号 ABIN6719689
发货至: 中国
  • 抗原 See all SPARCL1 ELISA试剂盒
    SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))
    适用
    • 6
    • 3
    • 2
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    46.9 pg/mL - 3000 pg/mL
    最低检测浓度
    46.9 pg/mL
    应用范围
    ELISA
    原理
    Sandwich ELISA for Quantitative Detection of Antigen
    样品类型
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    产品特性

    Synonyms: Ecm2, Extracellular matrix protein 2, Hevin, High endothelial venule protein, MAST 9, mast9, Matrix glycoprotein Sc1, PIG33, proliferation inducing protein 33, RAGS1 antigen, SC1, SPARC like 1, SPARC-like protein 1, Sparcl1, SPRL1_HUMAN

    Background: SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

    Gene Name: SPARCL1

    Production: Natural and recombinant human SPARCL1. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: I17-F664

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    Discover our top product SPARCL1 ELISA Kit
  • 应用备注

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 188pg/mL, 94pg/mL, 46.9pg/mL human SPARCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. We recommend that each human SPARCL1 standard solution and each sample is measured in duplicate. 2. Seal the plate with the cover and incubate at 37 °C for 90 min.

    ELISA Dilution: 46.9pg/mL-3000pg/mL

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • 抗原 See all SPARCL1 ELISA试剂盒
    SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))
    别名
    SPARCL1 (SPARCL1 产品)
    别名
    LOC445896 ELISA Kit, SPARCL1 ELISA Kit, sparcl ELISA Kit, sparcl1 ELISA Kit, DKFZp459I2327 ELISA Kit, Ecm2 ELISA Kit, Sc1 ELISA Kit, hevin ELISA Kit, mast9 ELISA Kit, PIG33 ELISA Kit, SC1 ELISA Kit, SPARCL-1 ELISA Kit, SPARCL1 ELISA Kit, SPARC like 1 ELISA Kit, SPARC-like 1 ELISA Kit, SPARC-like protein 1 ELISA Kit, LOC445896 ELISA Kit, SPARCL1 ELISA Kit, sparcl1 ELISA Kit, Sparcl1 ELISA Kit
    基因ID
    8404
    NCBI登录号
    NP_001121782
    UniProt
    Q14515
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