F11R ELISA 试剂盒
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- 抗原 See all F11R ELISA试剂盒
- F11R (F11 Receptor (F11R))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 46.9 pg/mL - 3000 pg/mL
- 最低检测浓度
- 46.9 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0813
- Supplier
- Rockland
- 原理
- Human JAM-A Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human JAM-A. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product F11R ELISA Kit
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- 应用备注
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ELISA_Dilution: 46.9pg/mL-3000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL human JAM-A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants or serum to each empty well. We recommend that each human JAM-A standard solution and each sample is measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: S28-V238
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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Development of a miniaturized 96-Transwell air-liquid interface human small airway epithelial model." in: Scientific reports, Vol. 10, Issue 1, pp. 13022, (2020) (PubMed).
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Development of a miniaturized 96-Transwell air-liquid interface human small airway epithelial model." in: Scientific reports, Vol. 10, Issue 1, pp. 13022, (2020) (PubMed).
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- 抗原 See all F11R ELISA试剂盒
- F11R (F11 Receptor (F11R))
- 别名
- JAM-A (F11R 产品)
- 背景
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Synonyms: CD 321, CD321, CD321 antigen, ESTM33, F11 receptor, F11R, JAM 1, JAM A, JAM, JAM-1, JAM-A, JAM1, JAM1_HUMAN, JAMA, JCAM, Jcam1, Junction adhesion molecule 1, Junction adhesion molecule mouse homolog of, Junctional adhesion molecule 1, Junctional adhesion molecule A, KAT, Ly106, PAM 1, PAM-1, PAM1, Platelet adhesion molecule 1, Platelet adhesion molecule, Platelet F11 receptor, PRO301, UNQ264, UNQ264/PRO301
Background: Junctional adhesion molecule A(JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.
- 基因ID
- 50848
- NCBI登录号
- NP_058642
- UniProt
- Q9Y624
- 途径
- Cell-Cell Junction Organization
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