IL1A ELISA 试剂盒
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- 抗原 See all IL1A ELISA试剂盒
- IL1A (Interleukin 1 alpha (IL1A))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 4.7 pg/mL - 300 pg/mL
- 最低检测浓度
- 4.7 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0208
- Supplier
- Rockland
- 原理
- Mouse IL-1 alpha Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant mouse IL-1 alpha. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 1 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product IL1A ELISA Kit
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.8pg/mL, 9.4pg/mL, 4.7pg/mL mouse IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. We recommend that each mouse IL-1 alpha standard solution and each sample is measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: S115-S270
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all IL1A ELISA试剂盒
- IL1A (Interleukin 1 alpha (IL1A))
- 别名
- IL-1 alpha (IL1A 产品)
- 背景
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Synonyms: Hematopoietin 1, IL 1 alpha, Il 1a, IL1, IL1 ALPHA, IL1A, IL1F1, Ilia, Interleukin 1 alpha, Interleukin 1 alpha precursor, Interleukin1 alpha, OTTHUMP00000203692, OTTMUSP00000016430, Preinterleukin 1, Pro interleukin 1, Pro interleukin 1 alpha, Prointerleukin 1, RP23-160G19.8
Background: Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18 kDa.
- 基因ID
- 16175
- NCBI登录号
- NP_034684
- UniProt
- P01582
- 途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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