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DKK1 ELISA 试剂盒

Rockland koa0561 DKK1 适用: 小鼠 Colorimetric Sandwich ELISA 46.9 pg/mL - 3000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
产品编号 ABIN6719676
Supplier Product No.: koa0561
发货至: 中国
  • 抗原 See all DKK1 ELISA试剂盒
    DKK1 (Dickkopf Homolog 1 (DKK1))
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    46.9 pg/mL - 3000 pg/mL
    最低检测浓度
    46.9 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0561
    Supplier
    Rockland
    原理
    Mouse DKK1 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant mouse DKK1. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 15 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product DKK1 ELISA Kit
  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse DKK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. We recommend that each mouse DKK1 standard solution and each sample is measured in duplicate.
    说明

    Standard: Expression system for standard: CHO, Immunogen sequence: S30-H272

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all DKK1 ELISA试剂盒
    DKK1 (Dickkopf Homolog 1 (DKK1))
    别名
    DKK1 (DKK1 产品)
    背景

    Synonyms: Dickkopf 1, Dickkopf homolog 1, Dickkopf related protein 1, DKK 1, hDkk 1, hDkk1, mDkk1, SK

    Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

    基因ID
    13380
    NCBI登录号
    NP_034181
    UniProt
    O54908
    途径
    WNT signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation
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