NGFB ELISA 试剂盒
-
- 抗原 See all NGFB ELISA试剂盒
- NGFB (Nerve Growth Factor beta (NGFB))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 产品特性
-
Synonyms: Beta nerve growth factor, Beta NGF, HSAN5, MGC161426, MGC161428, Nerve growth factor (beta polypeptide), Nerve growth factor beta polypeptide, Nerve growth factor beta subunit, NGFB, NID67
Background: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is human 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.
Gene Name: NGF
Production: Natural and recombinant human NGF. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: E.coli, Immunogen sequence: S122-A241
- Featured
- Discover our best selling NGFB ELISA Kit
- Top Product
- Discover our top product NGFB ELISA Kit
-
Nerve Growth Factor beta (NGFB) ELISA Kit
NGFB 适用: 人 Colorimetric Sandwich ELISA 6.86 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB 适用: 人 Colorimetric Sandwich ELISA 14-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nerve Growth Factor beta (NGFB) ELISA KitNGFB 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB 适用: 大鼠 Colorimetric Sandwich ELISA 39.063 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB 适用: 兔 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB 适用: 大鼠 Colorimetric Sandwich ELISA 15-15000 pg/mL Cell Culture Supernatant, Plasma, Serum
-
- 应用备注
-
Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human NGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants or serum to each empty well. It is recommended that each human NGF standard solution and each sample be measured in duplicate.
ELISA Dilution: 15.6pg/mL-1000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
-
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all NGFB ELISA试剂盒
- NGFB (Nerve Growth Factor beta (NGFB))
- 别名
- NGF - NGF beta (NGFB 产品)
- 别名
- NGFB ELISA Kit, ngfb ELISA Kit, ns:zft386 ELISA Kit, xx:zft386 ELISA Kit, zgc:109730 ELISA Kit, hsan5 ELISA Kit, beta-ngf ELISA Kit, Ngfb ELISA Kit, Beta-NGF ELISA Kit, HSAN5 ELISA Kit, beta-NGF ELISA Kit, nerve growth factor ELISA Kit, nerve growth factor b (beta polypeptide) ELISA Kit, beta-Nerve growth factor ELISA Kit, nerve growth factor (beta polypeptide) ELISA Kit, NGF ELISA Kit, ngfb ELISA Kit, FPV072 ELISA Kit, FPV076 ELISA Kit, ngf ELISA Kit, Ngf ELISA Kit
- 基因ID
- 4803
- NCBI登录号
- NP_002497
- UniProt
- P01138
- 途径
- NF-kappaB Signaling, RTK signaling, Regulation of Cell Size
-
