Dystroglycan IQ-ELISA 试剂盒
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- 抗原 See all Dystroglycan (DAG1) IQ-ELISA Kits
- Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))
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适用
- 人
- 检测方法
- qPCR
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Immunoquantitative (PCR-Based) Dystroglycan ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Semi-Quantitative
- 产品特性
- IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
- 组件
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- Dystroglycan Microplate (Item A): 96 well PCR plate coated with anti-Human Dystroglycan
- Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
- Standards (Item C): 2 vials of recombinant Human Dystroglycan
- Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
- Detection Affinity Reagent for Dystroglycan (Item F): 2 vials of a 4x concentrated solution of anti-Human Dystroglycan affinity reagent
- IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
- Primer Solution (Item I): 1.7 ml vial
- PCR Master Mix (Item J): 1.2 ml vial
- PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
- Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
- 试剂未包括
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- Real-time PCR instrument, Bio-Rad recommended
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for data analysis
- Tubes to prepare standard or sample dilutions
- Heating block or water bath capable of 80°C
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Dystroglycan 1 (DAG1) ELISA Kit
DAG1 适用: Various Species Colorimetric Competition ELISA 391 pg/mL - 100000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Dystroglycan 1 (DAG1) ELISA KitDAG1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Dystroglycan 1 (DAG1) ELISA KitDAG1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
Dystroglycan 1 (DAG1) ELISA KitDAG1 适用: 人 AA 30-653 Colorimetric Sandwich ELISA
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- 应用备注
- The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
- 说明
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The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.
- 样本量
- 25 µL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed
- Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
- Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
- Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
- Add 15μL Primer solution 10μL of PCR master mix to each well
- Run real-time PCR
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
- 有效期
- 6 months
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- 抗原 See all Dystroglycan (DAG1) IQ-ELISA Kits
- Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))
- 别名
- Dystroglycan (DAG1 产品)
- 别名
- LOC398500 IQ-ELISA Kit, dag1 IQ-ELISA Kit, MGC53537 IQ-ELISA Kit, 156DAG IQ-ELISA Kit, A3a IQ-ELISA Kit, AGRNR IQ-ELISA Kit, DAG IQ-ELISA Kit, MDDGC7 IQ-ELISA Kit, MDDGC9 IQ-ELISA Kit, DAG1 IQ-ELISA Kit, RAB7 IQ-ELISA Kit, dg IQ-ELISA Kit, a3a IQ-ELISA Kit, dag IQ-ELISA Kit, agrnr IQ-ELISA Kit, 156dag IQ-ELISA Kit, D9Wsu13e IQ-ELISA Kit, DG IQ-ELISA Kit, Dp427 IQ-ELISA Kit, Dp71 IQ-ELISA Kit, CG18250 IQ-ELISA Kit, CT41273 IQ-ELISA Kit, DmDG IQ-ELISA Kit, Dmel\\CG18250 IQ-ELISA Kit, atu IQ-ELISA Kit, dgn IQ-ELISA Kit, dys IQ-ELISA Kit, GB14967 IQ-ELISA Kit, APOJ IQ-ELISA Kit, CLI IQ-ELISA Kit, RATTRPM2B IQ-ELISA Kit, SGP-2 IQ-ELISA Kit, SGP2 IQ-ELISA Kit, SP-40 IQ-ELISA Kit, SP40 IQ-ELISA Kit, TRPM-2 IQ-ELISA Kit, TRPM2B IQ-ELISA Kit, Trpm2 IQ-ELISA Kit, Trpmb IQ-ELISA Kit, Ala-1 IQ-ELISA Kit, H9/25 IQ-ELISA Kit, Ly-27 IQ-ELISA Kit, Ly-6 IQ-ELISA Kit, Ly27 IQ-ELISA Kit, wu:fb83d06 IQ-ELISA Kit, wu:fi25f06 IQ-ELISA Kit, wu:fi37b08 IQ-ELISA Kit, zgc:109786 IQ-ELISA Kit, DystroGlycaN IQ-ELISA Kit, dystroglycan 1 L homeolog IQ-ELISA Kit, dystroglycan 1 IQ-ELISA Kit, dystroglycan 1 S homeolog IQ-ELISA Kit, Dystroglycan IQ-ELISA Kit, dystroglycan IQ-ELISA Kit, clusterin IQ-ELISA Kit, lymphocyte antigen 6 complex IQ-ELISA Kit, dgn-1 IQ-ELISA Kit, dag1.L IQ-ELISA Kit, dgn-2 IQ-ELISA Kit, dgn-3 IQ-ELISA Kit, DAG1 IQ-ELISA Kit, dag1.S IQ-ELISA Kit, Dag1 IQ-ELISA Kit, dag1 IQ-ELISA Kit, Dg IQ-ELISA Kit, LOC408826 IQ-ELISA Kit, Clu IQ-ELISA Kit, Ly6 IQ-ELISA Kit
- 基因ID
- 1605
- UniProt
- Q14118
- 途径
- Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus
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