MDM2 IQ-ELISA 试剂盒
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- 抗原 See all MDM2 IQ-ELISA Kits
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
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适用
- 人
- 检测方法
- qPCR
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Immunoquantitative (PCR-Based) MDM2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Semi-Quantitative
- 产品特性
- IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
- 组件
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- MDM2 Microplate (Item A): 96 well PCR plate coated with anti-Human MDM2
- Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
- Standards (Item C): 2 vials of recombinant Human MDM2
- Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
- Detection Affinity Reagent for MDM2 (Item F): 2 vials of a 4x concentrated solution of anti-Human MDM2 affinity reagent
- IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
- Primer Solution (Item I): 1.7 ml vial
- PCR Master Mix (Item J): 1.2 ml vial
- PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
- Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
- 试剂未包括
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- Real-time PCR instrument, Bio-Rad recommended
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for data analysis
- Tubes to prepare standard or sample dilutions
- Heating block or water bath capable of 80°C
- Top Product
- Discover our top product MDM2 ELISA Kit
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Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) ELISA Kit
MDM2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) ELISA KitMDM2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
- 说明
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The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.
- 样本量
- 25 µL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed
- Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
- Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
- Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
- Add 15μL Primer solution 10μL of PCR master mix to each well
- Run real-time PCR
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
- 有效期
- 6 months
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- 抗原 See all MDM2 IQ-ELISA Kits
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
- 别名
- MDM2 (MDM2 产品)
- 别名
- ACTFS IQ-ELISA Kit, HDMX IQ-ELISA Kit, hdm2 IQ-ELISA Kit, zgc:109834 IQ-ELISA Kit, 1700007J15Rik IQ-ELISA Kit, AA415488 IQ-ELISA Kit, Mdm-2 IQ-ELISA Kit, MDM2 proto-oncogene IQ-ELISA Kit, MDM2 proto-oncogene S homeolog IQ-ELISA Kit, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) IQ-ELISA Kit, MDM2 oncogene, E3 ubiquitin protein ligase IQ-ELISA Kit, transformed mouse 3T3 cell double minute 2 IQ-ELISA Kit, MDM2 IQ-ELISA Kit, mdm2.S IQ-ELISA Kit, Mdm2 IQ-ELISA Kit, mdm2 IQ-ELISA Kit
- 基因ID
- 4193
- UniProt
- Q00987
- 途径
- p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway
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