FLT4 IQ-ELISA 试剂盒
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- 抗原 See all FLT4 IQ-ELISA Kits
- FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))
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适用
- 小鼠
- 检测方法
- qPCR
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse Immunoquantitative (PCR-Based) VEGFR3 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Semi-Quantitative
- 灵敏度
- 0.02 ng/mL
- 产品特性
- IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
- 组件
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- VEGFR3 Microplate (Item A): 96 well PCR plate coated with anti-Mouse VEGFR3
- Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
- Standards (Item C): 2 vials of recombinant Mouse VEGFR3
- Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
- Detection Affinity Reagent for VEGFR3 (Item F): 2 vials of a 4x concentrated solution of anti-Mouse VEGFR3 affinity reagent
- IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
- Primer Solution (Item I): 1.7 ml vial
- PCR Master Mix (Item J): 1.2 ml vial
- PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
- Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
- 试剂未包括
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- Real-time PCR instrument, Bio-Rad recommended
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for data analysis
- Tubes to prepare standard or sample dilutions
- Heating block or water bath capable of 80°C
- Featured
- Discover our best selling FLT4 ELISA Kit
- Top Product
- Discover our top product FLT4 ELISA Kit
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Fms-Related Tyrosine Kinase 4 (FLT4) ELISA Kit
FLT4 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitHigh Sensitivity FLT4 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 大鼠 AA 25-770 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Serum
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 小鼠 AA 25-770 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Serum
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 人 Colorimetric Sandwich ELISA 15-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 小鼠 Colorimetric Sandwich ELISA 0.2-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
- 说明
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The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.
- 样本量
- 25 µL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed
- Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
- Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
- Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
- Add 15μL Primer solution 10μL of PCR master mix to each well
- Run real-time PCR
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
- 有效期
- 6 months
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- 抗原 See all FLT4 IQ-ELISA Kits
- FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))
- 别名
- VEGFR3 (FLT4 产品)
- 别名
- FLT41 IQ-ELISA Kit, LMPH1A IQ-ELISA Kit, PCL IQ-ELISA Kit, VEGFR3 IQ-ELISA Kit, AI323512 IQ-ELISA Kit, Chy IQ-ELISA Kit, Flt-4 IQ-ELISA Kit, VEGFR-3 IQ-ELISA Kit, Vegfr3 IQ-ELISA Kit, cb1059 IQ-ELISA Kit, fms related tyrosine kinase 4 IQ-ELISA Kit, FMS-like tyrosine kinase 4 IQ-ELISA Kit, fms-related tyrosine kinase 4 IQ-ELISA Kit, FLT4 IQ-ELISA Kit, Flt4 IQ-ELISA Kit, flt4 IQ-ELISA Kit
- 基因ID
- 14257
- UniProt
- P35917
- 途径
- RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling
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