Thyroxine T4 ELISA 试剂盒
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- 抗原 See all Thyroxine T4 (T4) ELISA试剂盒
- Thyroxine T4 (T4)
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适用
- 激素
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- Analytical Method
- Quantitative
- 产品特性
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ELISA kit for detection of Thyroxine in the research laboratory
Alternative Names: Free T4 ELISA Kit - Top Product
- Discover our top product T4 ELISA Kit
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- 应用备注
- Optimal conditions to be determined by end user
- 板类型
- Pre-coated
- 实验流程
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The CBI fT4 is a solid phase competitive ELISA. The samples, assay buffer and T4 enzyme conjugate are added to the wells coated with anti-T4 monoclonal antibody. fT4 in the patient's serum competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound fT4 and T4 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of fT4 in the samples. A standard curve is prepared relating color intensity to the concentration of fT4.
- 限制
- 仅限研究用
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- 注意事项
- All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, and HbsAg. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.
- 储存条件
- 4 °C
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- 抗原 See all Thyroxine T4 (T4) ELISA试剂盒
- Thyroxine T4 (T4)
- 别名
- Thyroxine (T4 产品)
- 物质类
- Amino Acid
- 背景
- The thyroid hormone, thyroxine (T4) is produced by the thyroid gland. An important component in the synthesis is iodine. The major form of thyroid hormone in the blood is thyroxine (T4). Thyroxine is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases (5'- iodinase). Thyroxine-binding globulin (TGB) is the major carrier protein for circulating thyroid hormone. Only a verysmall fraction of the circulating hormone is free (unbound) - T4 0.03 %.
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