HTRA2 ELISA 试剂盒
HTRA2
适用: 人
Colorimetric
Sandwich ELISA
0.2-50 ng/mL
Cell Culture Supernatant, Plasma, Serum
1 image
-
- 抗原 See all HTRA2 ELISA试剂盒
- HTRA2 (HtrA Serine Peptidase 2 (HTRA2))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.2-50 ng/mL
- 最低检测浓度
- 0.2 ng/mL
- 应用范围
- ELISA
- 原理
- Human HTRA2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detect human HtrA2. Other species not determined yet.
- 灵敏度
- 200 pg/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all HTRA2 ELISA试剂盒
- HTRA2 (HtrA Serine Peptidase 2 (HTRA2))
- 别名
- HTRA2 (HTRA2 产品)
- 别名
- CG8464 ELISA Kit, DmHtrA2 ELISA Kit, Dmel\\CG8464 ELISA Kit, HtrA2/Omi ELISA Kit, Omi ELISA Kit, dOmi ELISA Kit, domi ELISA Kit, htrA2 ELISA Kit, omi ELISA Kit, HTRA2 ELISA Kit, PRSS25 ELISA Kit, OMI ELISA Kit, PARK13 ELISA Kit, AI481710 ELISA Kit, Prss25 ELISA Kit, mnd2 ELISA Kit, HTRA2-related serine protease ELISA Kit, HtrA serine peptidase 2 ELISA Kit, HtrA2 ELISA Kit, HTRA2 ELISA Kit, htra2 ELISA Kit, Htra2 ELISA Kit
- 背景
-
Gene Names: HTRA2 OMI PRSS25
Protein names: Serine protease HTRA2, mitochondrial (EC 3.4.21.108) (High temperature requirement protein A2) (HtrA2) (Omi stress-regulated endoprotease) (Serine protease 25) (Serine proteinase OMI)
- 基因ID
- 27429
- UniProt
- O43464
- 途径
- Positive Regulation of Endopeptidase Activity
-
You are here:
