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TREM1 ELISA 试剂盒
TREM1
适用: 小鼠
Colorimetric
Sandwich ELISA
0.25-200 ng/mL
Cell Culture Supernatant, Plasma, Serum
TREM1ELISA Kit详情
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TREM1
(Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))
适用
All reactivities for TREM1 ELISA试剂盒
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.25-200 ng/mL
最低检测浓度
0.25 ng/mL
应用范围
ELISA
原理
Mouse TREM-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
样品类型
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
特异性
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
灵敏度
0.25 ng/mL
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples2 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
TREM1目标详情
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抗原
See all TREM1 ELISA试剂盒
TREM1
(Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))
别名
TREM-1 (TREM1 产品 )
别名
TREM1 ELISA Kit, CD354 ELISA Kit, TREM-1 ELISA Kit, triggering receptor expressed on myeloid cells 1 ELISA Kit, TREM1 ELISA Kit, Trem1 ELISA Kit, LOC100349504 ELISA Kit
背景
Gene Names: Trem1 Protein names: Triggering receptor expressed on myeloid cells 1 (TREM-1) (CD antigen CD354)
基因ID
58217
UniProt
Q9JKE2
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