MME ELISA 试剂盒
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- 抗原 See all MME ELISA试剂盒
- MME (Membrane Metallo-Endopeptidase (MME))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 61-15000 pg/mL
- 最低检测浓度
- 61 pg/mL
- 应用范围
- ELISA
- 原理
- Mouse Neprilysin ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes mouse Neprilysin.
- 灵敏度
- 61 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling MME ELISA Kit
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- Discover our top product MME ELISA Kit
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Membrane Metallo-Endopeptidase (MME) ELISA Kit
MME 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 人 AA 52-750 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 小鼠 AA 52-750 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
SensoLyte® 520 Neprilysin Activity Assay KitAct, FRET Fluorometric
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 人 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 小鼠 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all MME ELISA试剂盒
- MME (Membrane Metallo-Endopeptidase (MME))
- 别名
- Neprilysin (MME 产品)
- 别名
- CALLA ELISA Kit, CD10 ELISA Kit, NEP ELISA Kit, SFE ELISA Kit, 6030454K05Rik ELISA Kit, C85356 ELISA Kit, Nep ELISA Kit, neprilysin ELISA Kit, membrane metalloendopeptidase ELISA Kit, membrane metallo endopeptidase ELISA Kit, membrane metallo-endopeptidase ELISA Kit, MME ELISA Kit, Mme ELISA Kit
- 物质类
- Chemical
- 背景
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Gene Names: Mme
Protein names: Neprilysin (EC 3.4.24.11) (Atriopeptidase) (Enkephalinase) (Neutral endopeptidase 24.11) (NEP) (Neutral endopeptidase) (Skin fibroblast elastase) (SFE) (CD antigen CD10)
- 基因ID
- 17380
- UniProt
- Q61391
- 途径
- RTK signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration
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