XCL1 ELISA 试剂盒
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- 抗原 See all XCL1 ELISA试剂盒
- XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 150 pg/mL-50 ng/mL
- 最低检测浓度
- 150 pg/mL
- 应用范围
- ELISA
- 原理
- Human Lymphotactin (XCL1) ELISA Kit for cell culture supernatants and serum samples. EDTA is recommended as an anticoagulant for plasma in this array.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 灵敏度
- 150 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling XCL1 ELISA Kit
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- Discover our top product XCL1 ELISA Kit
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Chemokine (C Motif) Ligand 1 (XCL1) ELISA Kit
XCL1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 人 AA 22-114 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 小鼠 AA 22-114 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 小鼠 Colorimetric Sandwich ELISA 40-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 样品制备
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EDTA is recommended as an anticoagulant for plasma in this array.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all XCL1 ELISA试剂盒
- XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))
- 别名
- Lymphotactin (XCL1 产品)
- 别名
- ATAC ELISA Kit, LPTN ELISA Kit, LTN ELISA Kit, SCM-1 ELISA Kit, SCM-1a ELISA Kit, SCM1 ELISA Kit, SCM1A ELISA Kit, SCYC1 ELISA Kit, AI661682 ELISA Kit, Lptn ELISA Kit, Scyc1 ELISA Kit, Ltn ELISA Kit, XCL1 ELISA Kit, XCL2 ELISA Kit, X-C motif chemokine ligand 1 ELISA Kit, chemokine (C motif) ligand 1 ELISA Kit, cytokine SCM-1 beta ELISA Kit, lymphotactin ELISA Kit, XCL1 ELISA Kit, Xcl1 ELISA Kit, LOC490356 ELISA Kit, LOC100714756 ELISA Kit, LOC100349255 ELISA Kit
- 背景
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Gene Names: XCL1 LTN SCYC1
Protein names: Lymphotactin (ATAC) (C motif chemokine 1) (Cytokine SCM-1) (Lymphotaxin) (SCM-1-alpha) (Small-inducible cytokine C1) (XC chemokine ligand 1)
- 基因ID
- 6375
- UniProt
- P47992
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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