IL2 Receptor beta ELISA 试剂盒
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- 抗原 See all IL2 Receptor beta (IL2RB) ELISA试剂盒
- IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 22-6.000 pg/mL
- 最低检测浓度
- 22 pg/mL
- 应用范围
- ELISA
- 原理
- Human IL-2 R beta (CD122) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF,SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 灵敏度
- 22 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Interleukin 2 Receptor, beta (IL2RB) ELISA Kit
IL2RB 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, beta (IL2RB) ELISA KitIL2RB 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, beta (IL2RB) ELISA KitIL2RB 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples5-fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all IL2 Receptor beta (IL2RB) ELISA试剂盒
- IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))
- 别名
- IL-2 R beta (IL2RB 产品)
- 别名
- IL2RB ELISA Kit, il-2rb ELISA Kit, CD122 ELISA Kit, IL15RB ELISA Kit, P70-75 ELISA Kit, IL2RBC ELISA Kit, IL-15Rbeta ELISA Kit, IL15Rbeta ELISA Kit, Il-2/15Rbeta ELISA Kit, Il-2Rbeta ELISA Kit, p70 ELISA Kit, interleukin 2 receptor subunit beta ELISA Kit, interleukin-2 receptor subunit beta ELISA Kit, interleukin 2 receptor, beta ELISA Kit, interleukin 2 receptor, beta chain ELISA Kit, IL2RB ELISA Kit, LOC510185 ELISA Kit, il2rb ELISA Kit, Il2rb ELISA Kit
- 背景
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Gene Names: IL2RB
Protein names: Interleukin-2 receptor subunit beta (IL-2 receptor subunit beta) (IL-2R subunit beta) (IL-2RB) (High affinity IL-2 receptor subunit beta) (p70-75) (p75) (CD antigen CD122)
- 基因ID
- 3560
- UniProt
- P14784
- 途径
- JAK/STAT Signaling, Growth Factor Binding
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