Ectodysplasin A ELISA 试剂盒
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- 抗原 See all Ectodysplasin A (EDA) ELISA试剂盒
- Ectodysplasin A (EDA)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 10-2.500 pg/mL
- 最低检测浓度
- 10 pg/mL
- 应用范围
- ELISA
- 原理
- Human EDA-A2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-alpha, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Ectodysplasin A (EDA) ELISA Kit
EDA 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Ectodysplasin A (EDA) ELISA KitEDA 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Ectodysplasin A (EDA) ELISA KitEDA 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Ectodysplasin A (EDA) ELISA试剂盒
- Ectodysplasin A (EDA)
- 别名
- EDA-A2 (EDA 产品)
- 别名
- ECTD1 ELISA Kit, ED1 ELISA Kit, ED1-A1 ELISA Kit, ED1-A2 ELISA Kit, EDA-A1 ELISA Kit, EDA-A2 ELISA Kit, EDA1 ELISA Kit, EDA2 ELISA Kit, HED ELISA Kit, HED1 ELISA Kit, ODT1 ELISA Kit, STHAGX1 ELISA Kit, XHED ELISA Kit, XLHED ELISA Kit, si:ch73-223d24.5 ELISA Kit, Ed1 ELISA Kit, Eda-A1 ELISA Kit, Eda-A2 ELISA Kit, Ta ELISA Kit, tabby ELISA Kit, RGD1563178 ELISA Kit, ectodysplasin A ELISA Kit, ectodysplasin-A ELISA Kit, EDA ELISA Kit, eda ELISA Kit, Eda ELISA Kit
- 基因ID
- 1896
- UniProt
- Q92838
- 途径
- Tube Formation
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