TGFA ELISA 试剂盒
(8 references)-
- 抗原 See all TGFA ELISA试剂盒
- TGFA (Transforming Growth Factor, alpha (TGFA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 3-1000 pg/mL
- 最低检测浓度
- 3 pg/mL
- 应用范围
- ELISA
- 原理
- Human TGF alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ALCAM, Angiopoietin-1, Angiostatin, B7-1, BMP-7, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, HB-EGF, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-13 Ra1, IL-18 BPa, IL-18 Rb, IL-18, IL-2 Ra, MMP-1, IL-2 Rb, IL-2 Rg, IL-21 R, MMP-2 , MMP-3, MMP-10, MMP-13).
- 灵敏度
- 3 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling TGFA ELISA Kit
- Top Product
- Discover our top product TGFA ELISA Kit
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Transforming Growth Factor, alpha (TGFA) ELISA Kit
TGFA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitSmall Sample TGFA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: Cow Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 人 AA 40-89 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: Pig AA 40-89 Colorimetric Sandwich ELISA
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 兔 AA 40-89 Colorimetric Sandwich ELISA
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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: "GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands." in: Molecular cancer therapeutics, Vol. 15, Issue 2, pp. 251-63, (2016) (PubMed).
: "Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 103, Issue 5, pp. 1133-40, (2015) (PubMed).
: "Fetal concentrations of the growth factors TGF-α and TGF-β1 in relation to normal and restricted fetal growth at term." in: Cytokine, Vol. 60, Issue 1, pp. 157-61, (2013) (PubMed).
: "Erlotinib prolongs survival in pancreatic cancer by blocking gemcitabine-induced MAPK signals." in: Cancer research, Vol. 73, Issue 7, pp. 2221-34, (2013) (PubMed).
: "Inulin supplementation in rat model of pouchitis." in: Acta biochimica Polonica, Vol. 58, Issue 3, pp. 381-4, (2012) (PubMed).
: "Epidermal ADAM17 maintains the skin barrier by regulating EGFR ligand-dependent terminal keratinocyte differentiation." in: The Journal of experimental medicine, Vol. 209, Issue 6, pp. 1105-19, (2012) (PubMed).
: "Phase II study of panitumumab, oxaliplatin, 5-fluorouracil, and concurrent radiotherapy as preoperative treatment in high-risk locally advanced rectal cancer patients (StarPan/STAR-02 Study)." in: Annals of oncology : official journal of the European Society for Medical Oncology, Vol. 22, Issue 11, pp. 2424-30, (2011) (PubMed).
: "Comparison of three different regimens in the treatment of acute brucellosis: a multicenter multinational study." in: The Journal of antimicrobial chemotherapy, Vol. 23, Issue 3, pp. 433-9, (1989) (PubMed).
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: "GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands." in: Molecular cancer therapeutics, Vol. 15, Issue 2, pp. 251-63, (2016) (PubMed).
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- 抗原 See all TGFA ELISA试剂盒
- TGFA (Transforming Growth Factor, alpha (TGFA))
- 别名
- TGF-alpha (TGFA 产品)
- 别名
- TFGA ELISA Kit, LOC100009482 ELISA Kit, TGF alpha ELISA Kit, TGFA ELISA Kit, wa-1 ELISA Kit, wa1 ELISA Kit, RATTGFAA ELISA Kit, TGFAA ELISA Kit, NV15606 ELISA Kit, si:ch211-10e8.7 ELISA Kit, transforming growth factor alpha ELISA Kit, transforming growth factor alpha L homeolog ELISA Kit, transforming growth factor-alpha ELISA Kit, transforming growth factor, alpha ELISA Kit, TGFA ELISA Kit, TGF-A ELISA Kit, Tgfa ELISA Kit, tgfa.L ELISA Kit, LOC100121300 ELISA Kit, LOC100009150 ELISA Kit, tgfa ELISA Kit
- 背景
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Gene Names: TGFA
Protein names: Protransforming growth factor alpha [Cleaved into: Transforming growth factor alpha (TGF-alpha) (EGF-like TGF) (ETGF) (TGF type 1)]
- 基因ID
- 7039
- UniProt
- P01135
- 途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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