CCL20 ELISA 试剂盒
(6 references)-
- 抗原 See all CCL20 ELISA试剂盒
- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.5-600 pg/mL
- 最低检测浓度
- 1.5 pg/mL
- 应用范围
- ELISA
- 原理
- Human MIP-3 alpha (CCL20) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 灵敏度
- 1.5 pg/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling CCL20 ELISA Kit
- Top Product
- Discover our top product CCL20 ELISA Kit
-
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA Kit
CCL20 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: Pig Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 大鼠 AA 27-96 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 人 AA 27-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 小鼠 AA 28-97 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 20 (CCL20) ELISA KitCCL20 适用: 小鼠 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum
-
- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
-
: "The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery." in: International journal of cancer, Vol. 138, Issue 11, pp. 2688-99, (2016) (PubMed).
: "Adenosine Modulates NR4A Orphan Nuclear Receptors To Attenuate Hyperinflammatory Responses in Monocytic Cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 4, pp. 1436-48, (2015) (PubMed).
: "Involvement of corneal epithelial cells in the Th17 response in an in vitro bacterial inflammation model." in: Molecular vision, Vol. 19, pp. 85-99, (2013) (PubMed).
: "Macrophage inflammatory protein-3 alpha (MIP-3a) is a novel serum prognostic marker in patients with colorectal cancer." in: Journal of surgical oncology, Vol. 107, Issue 2, pp. 160-6, (2013) (PubMed).
: "Macrophage inflammatory protein-3alpha influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells." in: Toxicology, Vol. 249, Issue 2-3, pp. 116-22, (2008) (PubMed).
: "[Metal ceramic boundary surfaces in the Ni-Cr-ceramic system from a recent viewpoint]." in: Deutsche zahnärztliche Zeitschrift, Vol. 37, Issue 11, pp. 896-9, (1983) (PubMed).
-
: "The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery." in: International journal of cancer, Vol. 138, Issue 11, pp. 2688-99, (2016) (PubMed).
-
- 抗原 See all CCL20 ELISA试剂盒
- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
- 别名
- MIP-3 alpha (CCL20 产品)
- 别名
- MIP-3ALPHA ELISA Kit, CCL20 ELISA Kit, si:dkey-150o13.1 ELISA Kit, CKb4 ELISA Kit, LARC ELISA Kit, MIP-3-alpha ELISA Kit, MIP-3a ELISA Kit, MIP3A ELISA Kit, SCYA20 ELISA Kit, ST38 ELISA Kit, MIP-3A ELISA Kit, MIP-3[a] ELISA Kit, Scya20 ELISA Kit, exodus-1 ELISA Kit, C-C motif chemokine ligand 20 ELISA Kit, chemokine (C-C motif) ligand 20b ELISA Kit, chemokine (C-C motif) ligand 20 ELISA Kit, CCL20 ELISA Kit, ccl20b ELISA Kit, Ccl20 ELISA Kit
- 背景
-
Gene Names: CCL20 LARC MIP3A SCYA20
Protein names: C-C motif chemokine 20 (Beta-chemokine exodus-1) (CC chemokine LARC) (Liver and activation-regulated chemokine) (Macrophage inflammatory protein 3 alpha) (MIP-3-alpha) (Small-inducible cytokine A20) [Cleaved into: CCL20(1-67), CCL20(1-64), CCL20(2-70)]
- 基因ID
- 6364
- UniProt
- P78556
- 途径
- The Global Phosphorylation Landscape of SARS-CoV-2 Infection
-
